INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
capítulos de libros
Título:
Optimizing the bio-affinity interaction between His-tag proteins and Ni(II) modified substrates.
Autor/es:
VALENTI, LAURA E.; HERRERA, ELISA G.; STRAGLIOTTO, MA. FERNANDA; MARTINS, VITOR L.; TORRESI, ROBERTO M.; GIACOMELLI, CARLA E.
Libro:
Proteins at Interfaces III (ACS).
Editorial:
ACS
Referencias:
Año: 2012; p. 37 - 57
Resumen:
Optimizing the bio-affinity interactionbetween His-tag proteins and Ni(II) surfacesites.Laura E. Valentia, Elisa Herreraa, María Fernanda Stragliottoa, VitorL. Martinsb, Roberto M. Torresib, Carla E. Giacomellia,*a- Instituto de Fisicoquímica de Córdoba (INFIQC-CONICET).Departamento de Fisicoquímica. Facultad de Ciencias Químicas.Universidad Nacional de Córdoba. Ciudad Universitaria. 5000 Córdoba,Argentina.b- Instituto de Química - Universidade de São Paulo ? Av. Prof. LineuPrestes 748, 05508-000 São Paulo, SP, Brazil* Corresponding author. giacomel@fcq.unc.edu.arABSTRACT. The bio-affinity interaction between Histidine(His)-tag proteins and Ni(II) surface sites is exploited as abio-functionalization strategy to achieve a better surface bioactivitythan that provided by physical adsorption. Thisimproved functioning is mainly ascribed to the presence ofsite-oriented proteins on the surface, induced by theinteraction between the tag and the Ni(II) sites. In addition tothe induced bio-affinity interaction, His-tag proteins are alsospontaneously adsorbed (through hydrophobic andelectrostatic interactions) on the substrate. These physicallyadsorbed proteins are randomly oriented and less bio-active,lowering the surface bio-recognition capabilities. Therefore,the surface bio-functionalization based on His-Ni(II)interaction requires the optimization of the experimental conditions to promote the bio-affinity interaction whileminimizing physical adsorption. This optimization can beachieved by properly selecting the adsorption conditions(solution pH and ionic strength, protein surface coverage,etc.) and the washing agents prior to the detection of the biorecognitionevent. This chapter is aimed at discussingexperimental results related to the optimization of the bioaffinityinteraction between a particular recombinant His-tagantigen and Ni(II) surface sites.