Biomolecule and solid substrate interaction: Key factors in developing biofunctional surfaces

LAURA E. VALENTI; MARIA L. CAROT; CARLA E. GIACOMELLI

Encyclopedia of Surface and Colloid Science

Francis & Taylor

Año: 2011;

This chapter covers the different strategies used to biofunctionalize solid substrate with proteins and single strand short DNA (ss-DNA), with especial emphasis on the consequences of the adsorption process on the structure, orientation and biological activity of the biomolecules. To this end, the discussion is divided in view of the two selected biomolecules and the basic biofunctionalization strategies, given by physical and chemical adsorption, as reported in the last decade. Among the great variety of proposed strategies to achieve chemical adsorption of proteins, the discussion is limited to the most used procedures resulting in either randomly (exposed protein side chains on derivatized substrates) or site oriented (avidin-biotin, His tag proteins-metal cations, and protein A(G)-antibodies) adsorbed proteins. On the other hand, much less research has been done with ss-DNA adsorbed (either physically or chemically) on sorbent substrates. Therefore, the discussion is focused on the effect of the sorbent substrate and solution conditions on ss-DNA orientation and conformation. Finally, the most common strategy involving thiol-gold chemistry is also particularly addressed to summarize ss-DNA chemical adsorption. solution conditions on ss-DNA orientation and conformation. Finally, the most common strategy involving thiol-gold chemistry is also particularly addressed to summarize ss-DNA chemical adsorption. solution conditions on ss-DNA orientation and conformation. Finally, the most common strategy involving thiol-gold chemistry is also particularly addressed to summarize ss-DNA chemical adsorption.