INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Separation and Purification of Fuchsin Dyes Using Column Chromatography. Identification and Characterization
Autor/es:
M. N. MONTES DE OCA; I. M. AIASSA MARTINEZ; MARÍA N. URRUTIA; GERARDO A. ARGUELLO; C. S. ORTIZ
Revista:
JOURNAL OF CHROMATOGRAPHIC SCIENCE
Editorial:
PRESTON PUBL INC
Referencias:
Año: 2010 vol. 48 p. 618 - 623
ISSN:
0021-9665
Resumen:
Abstract New Fuchsin (NF), also known as Magenta III, has potential applications in photodynamic therapy. The commercial product labeled New Fuchsin contains two other dye components in different proportions, Magenta II and Magenta I (Rosaniline). The proportions of NF, Magenta II and Magenta I determined by RP-HPLC in the commercial sample used, were 71.6±0.4%, 25.2±0.2% and 2.8±0.1% (n=7), respectively. The isolation, purification and characterization of commercial NF dye components were carried out applying different techniques, such as preparative column liquid chromatography (PCLC), thin layer chromatography (TLC), reverse phase – high performance liquid chromatography (RP-HPLC), absorption spectrophotometry, nuclear magnetic resonance spectroscopy (NMR), electrospray ionization mass spectrometry (ESI-MS) and tandem electrospray ionization mass spectrometry (ESI-MS/MS). After separation and isolation, the degree of purity obtained for NF compound was higher than 95% and higher than 92% for Magenta II and Magenta I compounds. Therefore, it is essential to ensure a high degree of purity of these dyes as raw material to obtain new drugs intended for therapeutical treatments. Confirmation Fecha:  Thu, 4 Mar 2010 10:59:37 -0600 [04/03/10 13:59:37 ART] De:  Michael Graves <mgraves@prestonpub.com> Para:  gerardoa@fcq.unc.edu.ar Asunto:  Re: Manuscript status Hello Prof. Dr. Gerardo A. Argüello:We are in the process of finalizing your paper. It will be assigned a pageand issue number very soon.Thanks,Michael Graves-- Managing EditorJournal  of Chromatographic Science6600 W. Touhy AveNiles, IL 60714-4516Phone: 847-647-2900 ext. 1300Fax: 847-647-1155email: mgraves@prestonpub.comhttp://www.j-chrom-sci.com/http://mc.manuscriptcentral.com:80/jcs