INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Plasmonic sensing through bioconjugation of Ag nanoparticles: Towards the development of immunoassays for ultralow quantification of antigens in colloidal dispersions
Autor/es:
FRAIRE JUAN; MORTRICH RUBEN; MERCADAL PABLO; CORONADO EDUARDO
Revista:
Advanced materials letters
Editorial:
VBRI PRESS
Referencias:
Año: 2018 vol. 9 p. 456 - 461
ISSN:
0976-3961
Resumen:
The combination of the optical properties of silver nanoparticles with some of the tool-kits of the widespread used ELISAmethod for antigen quantification immunoassays, give rise to an enzyme free, low cost, fast and more sensitive opticalmethod denoted as Intensity Depletion Immuno-linked Assay (IDILA), that can be performed directly in colloidal dispersionswithout any immobilization of the capture antibody, antigen, secondary and primary antibodies on a substrate. Thecapabilities of the method for quantifying antigens at ultralow concentrations in colloidal dispersions as well as itsperformance are demonstrated for specific antigens of importance in medicine and in food science.