CONICET | Buscador de Institutos y Recursos Humanos

This work describes the advantages of carbon nanotubes paste electrodes (CNTPE) modified with a melanic polymer for the highly sensitive and selective quantification of dopamine. The polymer is electrogenerated from a 3.0×10−3M l-dopa solution (in a 0.050M phosphate buffer pH 7.40) by applying 0.80V for 120 min. Two strategies for dopamine quantification are described, the amperometric detection at 0.200V using 1.0×10−3M ascorbic acid in the measurement solution to improve the sensitivity of the assay; and the adsorptive stripping detection with medium exchange using differential pulse voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. of the assay; and the adsorptive stripping detection with medium exchange using differential pulse voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 0.80V for 120 min. Two strategies for dopamine quantification are described, the amperometric detection at 0.200V using 1.0×10−3M ascorbic acid in the measurement solution to improve the sensitivity of the assay; and the adsorptive stripping detection with medium exchange using differential pulse voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. of the assay; and the adsorptive stripping detection with medium exchange using differential pulse voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. 5.0×10−5M dopac was really negligible in both cases, demonstrating the advantages of the melanic polymer as permselective layer. polymer as permselective layer. ×10−3M l-dopa solution (in a 0.050M phosphate buffer pH 7.40) by applying 0.80V for 120 min. Two strategies for dopamine quantification are described, the amperometric detection at 0.200V using 1.0×10−3M ascorbic acid in the measurement solution to improve the sensitivity of the assay; and the adsorptive stripping detection with medium exchange using differential pulse voltammetry (DPV) as transduction mode. Detection limits of 2.0nM and 20nM were obtained with amperometry and adsorptive stripping, respectively. The interference of 5.0×10−4M ascorbic acid and 5.0×