INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Characterization of DNA layers adsorbed on glassy carbon electrodes
Autor/es:
MARÍA L PEDANO; LÍA I. PIETRASANTA; MANUEL LÓPEZ TEIJELO; GUSTAVO A. RIVAS
Revista:
ELECTROANALYSIS
Editorial:
Wiley-VCH
Referencias:
Año: 2008 vol. 20 p. 739 - 749
ISSN:
1040-0397
Resumen:
A DNA layer adsorbed at glassy carbon electrodes (GCE) was characterized by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of the adsorbed DNA layer on polished glassy carbon electrodes was assessed indirectly by ellipsometric measurements. Ellipsometry was also useful to evaluate the influence of the oxide layer formed on glassy carbon electrodes, either spontaneously or after electrochemical pretreatments, on the DNA adsorption and further electrooxidation process. SEM and AFM images of the electrode surface covered by a thick layer of DNA reveal a nonuniform distribution, leaving channels and islands of the biological material.