Separation and Purification of Fuchsin Dyes Using Column Chromatography. Identification and Characterization

M. N. MONTES DE OCA; I. M. AIASSA MARTINEZ; MARÍA N. URRUTIA; GERARDO A. ARGUELLO; C. S. ORTIZ

JOURNAL OF CHROMATOGRAPHIC SCIENCE

Año: 2008

0021-9665

New Fuchsin (NF), also known as Magenta III, has potential applications in photodynamic therapy. The commercial product labeled New Fuchsin contains two other dye components in different proportions, Magenta II and Magenta I (Rosaniline). The proportions of NF, Magenta II and Magenta I determined by RP-HPLC in the commercial sample used, were 71.6±0.4%, 25.2±0.2% and 2.8±0.1% (n=7), respectively. The isolation, purification and characterization of commercial NF dye components were carried out applying different techniques, such as preparative column liquid chromatography (PCLC), thin layer chromatography (TLC), reverse phase – high performance liquid chromatography (RP-HPLC), absorption spectrophotometry, nuclear magnetic resonance spectroscopy (NMR), electrospray ionization mass spectrometry (ESI-MS) and tandem electrospray ionization mass spectrometry (ESI-MS/MS). After separation and isolation, the degree of purity obtained for NF compound was higher than 95% and higher than 92% for Magenta II and Magenta I compounds. Therefore, it is essential to ensure a high degree of purity of these dyes as raw material to obtain new drugs intended for therapeutical treatments.