Lyophilized Rhodotorula yeast as all-in-one redox biocatalyst: Access to enantiopure building blocks by simple chemoenzymatic one-pot procedures

CELESTE AGUIRRE PRANZONI; FABRICIO R. BISOGNO; ALEJANDRO A. ORDEN; MARCELA KURINA-SANZ

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 114 p. 19 - 24

1381-1177

Rhodotorula sp. LSL, isolated from a local landfarming was able to catalyze the reduction of prochiral arylketones into sec-alcohols with excellent Prelog stereoselectivity (ee > 99%). The use of resting and lyophilized cells was optimized accessing to an easy-to-use whole cell biocatalyst that efficiently works even under non-sterile conditions, without the addition of external cofactors and using plain water as solvent. The catalyst resistance in alkaline media enabled (chemo)enzymatic one-pot procedures at high pH values. So a simple and efficient methodology was applied to prepare alternatively enantiopure β-halohydrins, terminal diols and epoxides from aromatic α-haloketones.