INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Dynamics of One-State Downhill Protein Folding
Autor/es:
PENG LI; FABIANA Y. OLIVA; ATHI NAGANATHAN; VICTOR MUÑOZ
Revista:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Editorial:
NATL ACAD SCIENCES
Referencias:
Lugar: Washington DC; Año: 2009 vol. 106 p. 103 - 108
ISSN:
0027-8424
Resumen:
The small helical protein BBL has been shown to fold and unfold in
the absence of a free energy barrier according to a battery of
quantitative criteria in equilibrium experiments, including probedependent
equilibrium unfolding, complex coupling between denaturing
agents, characteristic DSC thermogram, gradual melting
of secondary structure, and heterogeneous atom-by-atom unfolding
behaviors spanning the entire unfolding process. Here, we
present the results of nanosecond T-jump experiments probing
backbone structure by IR and end-to-end distance by FRET. The
folding dynamics observed with these two probes are both exponential
with common relaxation times but have large differences
in amplitude following their probe-dependent equilibrium unfolding.
The quantitative analysis of amplitude and relaxation time
data for both probes shows that BBL folding dynamics are fully
consistent with the one-state folding scenario and incompatible
with alternative models involving one or several barrier crossing
events. At 333 K, the relaxation time for BBL is 1.3s, in agreement
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
s, in agreement
with previous folding speed limit estimates. However, late folding
events at room temperature are an order of magnitude slower (20
s), indicating a relatively rough underlying energy landscape. Our
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.
s), indicating a relatively rough underlying energy landscape. Our
results in BBL expose the dynamic features of one-state folding and
chart the intrinsic time-scales for conformational motions along the
folding process. Interestingly, the simple self-averaging folding
dynamics of BBL are the exact dynamic properties required in
molecular rheostats, thus supporting a biological role for one-state
folding.