The adsorption-desorption process of bovine serum albumin on carbon nanotubes

LAURA E. VALENTI; PABLO A. FIORITO; CARLOS D. GARCIA; CARLA EUGENIA GIACOMELLI

JOURNAL OF COLLOID AND INTERFACE SCIENCE

Elsevier

Año: 2007 vol. 307 p. 349 - 356

0021-9797

The aim of this work is to study the adsorption–desorption process of bovine serum albumin (BSA) on carbon nanotubes (CNT) by reflectometry. The effect of the surface properties was analyzed by comparing the behavior of BSA on silica. The experiments were performed by reflectometry at different BSA concentrations, at pH 3.0, 4.8, and 7.0 and at two ionic strengths. Protein desorption was induced by either dilution with buffer or the addition of SDS. The initial adsorption rate is controlled by the attachment of BSA molecules to the surface, and strongly diminishes at pH 7. Adsorption isotherms reflect the high affinity of BSA for both sorbent surfaces and reach well-defined plateau values that depend on the pH, being the highest at pH 4.8 on CNT. Experiments performed at different ionic strengths (NaCl added) showed a less pronounced effect. Dilution does not induce desorption on either surface however, the addition of SDS removes protein only from the silica surface.