Ferrocenyl-modified hyperbranched poly(ethylenimine)as platform for the immobilization of gold nanoparticles and redox enzymes

BRACAMONTE V.; GUSTAVO RIVAS; FERREYRA, N.F

JOURNAL OF ELECTROANALYTICAL CHEMISTRY - (Print)

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2014 vol. 721 p. 124 - 131

1572-6657

In this work we describe the synthesis and characterization of a new ferrocenyl-modified hyper branched poly(ethylenimine) and its application for immobilization of gold nanoparticles and redox enzymes. The effects of the supporting electrolyte and ionic strength in the electrochemical behavior of redox polymer were studied by electrochemical quartz crystal microbalance (EQCM). The interactions of the polymer with the anions from the supporting electrolyte, incorporated during the redox process, determined its electrochemical behavior. The polymer was employed for the immobilization of gold nanoparticles and the enzymes glucose oxidase (GOx) on the top of gold thiolated surfaces. The layer-by-layer assembly was evaluated by UV-visible spectrophotometry, surface plasmon resonance (SPR) and electrochemical techniques following the signal of the redox centers. The ferrocenyl-modified polymer was an efficient platform to the immobilization of both: metallic nanoparticles and enzymes.