Study on the interaction of Basic Violet 2 with Hydroxypropyl-â-cyclodextrin

AIASSA MARTINEZ, IVANA M.; MONTES DE OCA, MARIA N; IRIARTE, ANA G; ORTIZ, CRISTINA S; ARGUELLO GERARDO A

DYES AND PIGMENTS

ELSEVIER SCI LTD

Año: 2011 vol. 92 p. 758 - 765

0143-7208

The formation of the inclusion complexes of Basic Violet 2 (New Fuchsin) with hydroxypropyl-B-cyclodextrin (HP-B-CD) was studied by UV-Vis absorption and fluorescence spectroscopy. The binding constants of the inclusion complexes, KB, were obtained considering the maximum shift of the absorption spectra, the absorption areas deconvoluted as the sum of two gaussian constituents, the relative fluorescence quantum yield and the fluorescence maximum shift. The values achieved were 2.7 x103 M-1, 0.95 x103 M-1 and 2.8 x 103 M-1, 2.2 x 103 M-1, respectively. Ab-initio calculations using B3LYP/6-31G level of approach and the natural bond orbital (NBO) analysis were performed to gain a deeper understanding of the inclusion phenomena. The result showed a high complexation in aqueous media.B-cyclodextrin (HP-B-CD) was studied by UV-Vis absorption and fluorescence spectroscopy. The binding constants of the inclusion complexes, KB, were obtained considering the maximum shift of the absorption spectra, the absorption areas deconvoluted as the sum of two gaussian constituents, the relative fluorescence quantum yield and the fluorescence maximum shift. The values achieved were 2.7 x103 M-1, 0.95 x103 M-1 and 2.8 x 103 M-1, 2.2 x 103 M-1, respectively. Ab-initio calculations using B3LYP/6-31G level of approach and the natural bond orbital (NBO) analysis were performed to gain a deeper understanding of the inclusion phenomena. The result showed a high complexation in aqueous media.