Unraveling the spatial distribution of immunoglobulins, enzymes and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies

N.F. FERREYRA*, E.S. FORZANI, M. LÓPEZ TEIJELO, L. GUÉRENTE, P. LABBÉ

LANGMUIR

Año: 2006 vol. 22 p. 8931 - 8938

0743-7463

The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ã-¢ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.