INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Supramolecular analytical chemistry: spectrofluorimetric determination of 6- hydroxymelatonin with hydroxypropyl-beta-cyclodextrin.
Autor/es:
A. GUILLERMO BRACAMONTE; GUADALUPE G. MIÑAMBRES ; ALICIA V. VEGLIA
Revista:
ARKIVOC - ONLINE JOURNAL OF ORGANIC CHEMISTRY
Editorial:
ARKAT USA INC
Referencias:
Lugar: Florida; Año: 2011 vol. vii p. 439 - 449
ISSN:
1551-7004
Resumen:
The effect of the addition of native (a, b and g) and derivative (methyl-b and hydroxypropyl-b) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-b-CD (HPCD) showed the maximum enhancement and the association constant (KA, mol-1 L) was determined (60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free substrate (f6HMCD/f6HM) = 6.0±0.2 provides analytical advantages. On the basis of this supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of the method was performed in urine samples with very good recoveries 95-109%.