INFIQC 05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Adsorption and electrooxidation of DNA at glassy carbon paste electrodes
Autor/es:
M. L. PEDANO AND G. A. RIVAS
Revista:
ANALYTICAL LETTERS
Editorial:
Taylor & Francis
Referencias:
Lugar: Londres; Año: 2010 vol. 43 p. 1703 - 1712
ISSN:
0003-2719
Resumen:
This work reports the study of the adsorption and electrooxidation of nucleic acids on glassy carbon paste electrodes (GCPE) by using chronopotentiometric stripping analysis. The influence of electrochemical pretreatments, binder content, supporting electrolyte, accumulation potential and time on the adsorption and further electrooxidation of oligo(dG)21is discussed. The adsorption behavior of double stranded calf thymus DNA (dsDNA) on GCPE is also evaluated. Trace (ppb) levels of oligo(dG)21 and (ppm) levels of dsDNA can be readily detected following short accumulation periods, with detection limits of 21 ppb and 200ppb for oligo(dG)21 and dsDNA, respectively. The confined DNA layer demonstrated to be stable in air, in 0.200 M acetate buffer pH 5.00 and in 0.020 M phosphate buffer pH 7.40 + 0.50 M NaCl.
