Effect of the association of reduced gluthatione and ciprofloxacin in the antimicrobial activity in Staphylococcus aureus

PÁEZ PAULINA LAURA; BECERRA MARÍA CECILIA; ALBESA INÉS

FEMS MICROBIOLOGY LETTERS

Blackwell Science

Lugar: England; Año: 2010 vol. 303 p. 101 - 105

0378-1097

We report the effect of GSH and the role of reactive oxygen species (ROS), assayed by Nitro Blue Tetrazolium reaction, on the antibacterial action of ciprofloxacin (CIP), gentamicin (GEN) and chloramphenicol (CMP) in Staphylococcus aureus 22 resistant to CIP and GEN, and in S. aureus ATCC 29213 sensitive to the above three antibiotics. The association of glutathione (GSH) with CIP or GEN significantly reduced the value of the minimum inhibitory concentration (MIC) in resistant S. aureus 22, measured by the macrodilution method, with a concomitant increase of intracellular ROS and a decrease of extracellular ROS. However, GSH did not provoke modifications in MIC or ROS generated by CMP. Furthermore, in the sensitive S. aureus ATCC 29213, the association of GSH with CIP, GEN or CMP did not generate any significant variations of MIC or ROS. There was a correlation between the stimulus of intracellular ROS and the decrease of MIC caused by exogenous GSH. According to the results obtained, it is possible to modify the sensitivity of resistant strains of S. aureus by the addition of exogenous GSH.