IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Phototherapeutic Studies of Rubiadin: Anthraquinone Photosensitizer Isolated from Heterophyllaea pustulata (Rubiáceae).
Autor/es:
COMINI L. R; FERNÁNDEZ I.; RUMIE VITTAR B.; NÚÑEZ MONTOYA S. C.; CABRERA J. L.; RIVAROLA V.
Lugar:
Córdoba
Reunión:
Congreso; 16th INTERNACIONAL CONGRESS OF PHOTOBIOLOGY; 2014
Resumen:
Rubiadin is an anthraquinone isolated from the phototoxic shrub, Heterophyllaea pustulata Hook f. We demonstrated that this AQ is photosensitizer Type I and Type II 1. Considering that an important research line, studies photosensitizing compounds and their uses in photodynamic therapy (PDT), the photodynamic activity of Rubiadin in vitro was evaluated with satisfactory results 2. The next step was to evaluate their photodynamic activity in vivo, which is the aim of this work. Therefore, the pharmacokinetic, acute toxicity and phototherapeutic studies were assayed in a Balb/C mouse cancer model. Pharmacokinetic studies were carried out by intraperitoneal injection (i.p.) of Rubiadin (80 mg/kg). Then, this compound was recovered from serum and several organs at different times and analyzed by HPLC. Acute toxicity signs were evaluated after the i.p. injection of the same concentration of Rubiadin. The values of physiological parameters (creatinine, urea and transaminase), were analyzed in serum of control animals and those AQ-treated at different times. For phototherapeutic studies, an actinic lamp at 2 J/cm2 was used as light source for each session (24 and 48 h) after administration of a single dose of this AQ (80 mg/Kg). Rubiadin efficiency was evaluated through the tumor growth curves during 10 days after a PDT-treatment. Pharmacokinetic studies showed that AQ levels in liver, skin and tumor were significant at 24 h after administration, and disappeared at 7 days post-administration. In addition, at the times tested, not significant differences were observed in values of physiological parameters between the AQs-treated mice vs. controls. The PDT-treated tumors showed a delay in growth rate compared to untreated control mice. 1 S.C. Núñez Montoya, L.R. Comini, M. Sarmiento, M.C. Becerra, I. Albesa, Gustavo A. Argüello, J.L. Cabrera. J.Photochem.Photobiol. B:Biol. 2005,78,77. 2 L. Comini, I. Fernandez, N.B. Rumie Vittar, S.C. Nuñez Montoya, J.L. Cabrera, V. Rivarola. Phytomed. 2011,18,1093.