IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Bacterial biofilm evidence in nasal polyposis.
Autor/es:
MARIO E. ZERNOTTI; NATALIA ANGEL VILLEGAS; MAGDALENA ROQUES REVOL; CARLOS E. BAENA-CAGNANI; JULIO E. ARCE MIRANDA; MARISA E. PAREDES; INÉS ALBESA; MARÍA G. PARAJE.
Revista:
JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY
Editorial:
Esmon Publicidad, S.A.
Referencias:
Lugar: España; Año: 2010 vol. 20 p. 380 - 385
ISSN:
1018-9068
Resumen:
 Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium. Biofilms were demonstrated in vivo in two patients and no biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of one more inflammatory chronic factor, the bacterial biofilms, which can contribute to the damage of the nasal mucosae, to the increase of inflammatory cells in the tissue and to the subsequent hyperplasic process.  Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been involved in many inflammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas and more recently in CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities developing biofilms. Objectives: The aim of this study is to determine and characterize the presence of biofilms in CRS/NP. Methods: A prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. In ten patients without CRS/NP septoplasty was perform and were included as control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typified and the material was examined in vitro by a spectrophotometer, and in vivo by means of optical microscopy and confocal scanning laser microscopy. Results: A moderate to high ability to form in vitro biofilms was found in 9 out of 12 patients with CRS/NP (values between 0.284±0.017 and 3.337±0.029 optical density). The isolated microorganisms were Staphylococcus in five patients, Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corinebacterium.