IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
PRENYLATED FLAVONOID 6PP SHOWS DUAL ACTION: ANTIFUNGAL EFFECT AND RHODAMINE 6G EFFLUX INHIBITION
Autor/es:
PERALTA MARIANA A; CALISE MAXIMILIANO; ORTEGA, M. GABRIELA; CABRERA, JOSE L.; DIEZ, R.A.; PEREZ, CRISTINA
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Año: 2009 vol. 33 p. 11 - 11
ISSN:
0327-9545
Resumen:
Some compounds of vegetal origin show antimicrobial synergism. When 2´,4´-dihydroxy-5´-(1´´´,1´´´-dimethylallyl)-6-prenyl-pinocembrin (6PP, isolated from roots of Dalea elegans) and fluconazole (Flz) were incorporated to the incubation medium, inhibited the efflux of rhodamine 6G (Rh 6G) in Candida albicans resistant to azoles (CaR). This strain, isolated from the oral cavity, was obtained from Dr. T.White (University of Washington, Seattle, EE.UU) and expresses transporters of CDR1, CDR2 and MDR1 type. Our parameters were for 6PP and Flz respectively, maximum inhibition: 45.46% and 41.42%; apparent KI 42.95 µM and 107.81 µM.  The addition of Flz in culture medium of CaR was studied on the cellular growth (A), Rh 6G efflux (B) and antifungal activity (C). At concentrations 0; 3,2; 6,5 and 13 µM  of Flz, respectively, were detected the following % values: 100, 114, 114 and 109, for A (absorbance to 580 nm); 70, 48, 52 and 68, for B (retention of fluorescence, 100 in efflux reversion by  1,000 µM 6PP). In antifungal activity evaluation (C), 20 mM 6PP inhibited fungal growth at 0 (23 mm diameter) and 13 µM Flz (13 mm). The results are consistent with the expression of constitutive efflux pumps and a dual action of 6PP: inhibition of azole transporters and antifungal effect on CaR