IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Biosynthesized silver nanoparticles: decoding their mechanism of action in S. aureus and E. coli.
Autor/es:
VIVIANA CANO ARISTIZÁBAL; MELISA DE LOS ANGELES QUINTEROS; PAULINA L PÁEZ; MELISA DE LOS ANGELES QUINTEROS; PAULINA L PÁEZ; MARIA GABRIELA PARAJE; MARIA GABRIELA PARAJE; VIVIANA CANO ARISTIZÁBAL
Revista:
Journal of Clinical Toxicology
Editorial:
Journal of Clinical Toxicology
Referencias:
Año: 2017 vol. 07
Resumen:
The generation of oxidative stress in bacteria by the presence of silver nanoparticles (AgNPs) is already widely known1,2. If the cell cannot respond to oxidative injury produced by increased species reactive oxygen (ROS),the oxidation of macromolecules such as proteins, lipids and DNA occurs, leading to the death of the bacterium3,4. In previous results (Quinteros et al. 5), we observed as biosynthesized AgNPs that had antibacterial activity, generated an increase of ROS and RNI in Staphylococcus aureus, Escherichia coli andPseudomonas aeruginosa, being responsible for their toxicity and bacterial death. According to the above, we set out to delve into the mechanism of action of AgNPs, by determining markers of oxidative stress, such as protein oxidation, lipids, DNA and changes in membrane potential in two reference strains S. aureus ATCC 29213 and E. coli ATCC 25922. We found that theincrease in the levels of ROS is associated with the oxidation of different macromolecules important for the normal functioning of the cell,so that oxidative stress would be one of the mechanisms by which the AgNPs would exert their toxicity in these two strains of great clinical relevance. In this way, we are making a great contribution on the toxicity produced by AgNPs.