IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Histopathology of roots of three tomato cultivars infected with two separate isolates of the false root-knot nematode Nacobbus aberrans
Autor/es:
CABRERA V. A.; N. DOTTORI; DOUCET MARCELO
Revista:
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017 vol. 148 p. 393 - 403
ISSN:
0929-1873
Resumen:
The plant-parasitic nematode Nacobbus aberrans attacks weeds and cultivated plants, causing drastic crop yield losses. Several tomato cultivars, such as Superman and Mykonos, are produced by Seminis® as nematode resistant and are widely used in Argentina;their specific resistance response to different nematode species, however, is still unknown. In this study we explored the response of tomato cultivars to the infection of N. aberrans isolates, and determined the host status by performing histological analyses and estimating egg mass index (EMI). Two Argentina isolates (from Lules-Tucumán and Río Cuarto-Córdoba) were tested separately in plants of Superman, Mykonos and Platense cultivars. Plants were maintained in a greenhouse for 90 days; then EMI was estimated in root systems and the material was processed to prepare histological slides and for histochemical test. Infected roots exhibited galls with females and a syncytium (feeding site) developed inside them. The vascular tissues were disorganized and displaced to the periphery; xylem percentage was lower than that in the control plants. All the cultivars were susceptible and developed a close plant parasite relationship, with Mykonos and Platense cultivars infected with the Lules isolate being the most highly affected, as indicated by their highest EMI values. Superman was the least susceptible cultivar, as evidenced by its lowest EMI values, the amount of starch observed, the presence of thickened cell walls around the nematode and the egg mass, and the low percentage of gall occupied by syncytium.