IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Effect of luteolin on antioxidant activity of superoxide dismutase and catalase altered by gentamicin in human leukocytes
Autor/es:
BUSTOS, PAMELA SOLEDAD; ORTEGA, MARÍA GABRIELA; PÁEZ, PAULINA LAURA; CABRERA, JOSÉ LUIS
Revista:
MEDICINA (BUENOS AIRES)
Editorial:
MEDICINA (BUENOS AIRES)
Referencias:
Lugar: Buenos Aires; Año: 2016 vol. 76
ISSN:
0025-7680
Resumen:
Superoxide dismutase (SOD) and catalase (CAT) are important endogenous antioxidant defenses against attack by reactive oxygen species (ROS). SOD, dismutes to superoxide anion in oxygen and hidrogen peroxide, while CAT catalyzes the decomposition of hidrogen peroxide into oxygen and water. Gentamicin (GEN) is an antibiotic whose clinical usefulness is limited by the development of side effects that would be associated with increased oxidative stress in human cells. Previous studies in our group demonstrated that GEN is able to induce ROS production and alter the activity of these enzymes in mononuclear (MN) and polymorphonuclear (PMN) human leukocytes. In order to find natural compounds that neutralize the leukotoxicity of GEN, we evaluated the effect of luteolin (L), a flavonoid isolated from fruits of Prosopis strombulifera var. strombulifera with antioxidant activity, as potential protective agent against oxidative stress induced by GEN. The SOD activity was evaluated by assaying Methionine, Riboflavin and NBT, and the CAT activity, using potassium dichromate in acetic acid, both assays by spectrophotometry.GEN, in MN induced an increase of the activity of both enzymes SOD and CAT at 0.25 and 8 µg/mL, as a response that counteracts the ROS induced by the antibiotic, while at 128 and 256 µg/mL occurs a decrease of enzyme activity, possibly due to exhaustion produced by the exacerbated generation of ROS. On the other hand, in PMN, GEN caused a decrease of enzyme activity at all concentrations tested. As for the effect of L it was observed that luteolin (0.08, 0.25 and 0.8 µM in MN and 0.4, 1.7 and 6.8 µM in PMN) tends to level the antioxidant activity of SOD and CAT at similar values to basal level, especially at higher concentrations. Therefore, L shown ROS scavenger effect and contribute to the activity of endogenous antioxidant defenses, demonstrating a marked protective activity of L against oxidative stress generated by GEN in human leukocytes.