Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents

AIASSA MARTINEZ IM; DALMASSO PR; QUINTEROS MA; PÁEZ PL

International Journal of Biomaterials

Hindawi Publishing Corporation

Año: 2016 vol. 2016 p. 1 - 7

Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobialproperties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonasaeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37∘C with a silvernitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UVvisspectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudosphericalnanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of AgNPs.Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic andopportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Proteus mirabilis,Acinetobacter baumannii, Escherichia coli, P. aeruginosa, and Klebsiella pneumonia. Ag-NPs were found to be bioactive at picomolarconcentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This workdemonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenicmultidrug-resistant bacteria such as methicillin-resistant S. aureus, A. baumannii, and E. coli. In addition, these Ag-NPs showednegligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.