IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Hemolysin from Escherichia coli induces oxidative stress in blood
Autor/es:
JOSÉ LUIS BARONETTI, NATALIA ANGEL VILLEGAS, VIRGINIA AIASSA, MARÍA GABRIELA PARAJE AND INÉS ALBESA
Revista:
TOXICON
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 70 p. 15 - 20
ISSN:
0041-0101
Resumen:
Hemolysin (HlyA) produced by some stains of Escherichia coli is considered to be an
important virulence factor of those bacteria. On the other hand, reactive oxygen species
(ROS) have been reported to be involved in the pathogenesis of different diseases via
oxidative stress generation. The purpose of this study was to analyze the capacity of HlyA
to induce oxidative stress in whole blood cultures (WBCs). To this end, ROS production, the
damage induced in lipids and proteins, and the antioxidant defense system was evaluated
in blood cultures exposed to low concentrations of HlyA.We found that HlyA increased the
level of free radicals detected by chemiluminescence assay. Moreover, lipid peroxidation
and protein damage was significantly increased in cultures treated with HlyA in comparation
with those found in control cultures. On the other hand, a decrease in total
antioxidant capacity of plasma and in the activity of superoxide dismutase (SOD) was
observed in plasma from blood treated with HlyA. Collectively, our data demonstrate that
low concentrations of E. coli hemolysin induced oxidative stress in WBCs with the induction
of different oxidative damage biomarkers.