IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
PHYTOTOXICITY OF SECONDARY METABOLITES ISOLATED FROM Flourensia oolepis S.F.BLAKE
Autor/es:
DIAZ NAPAL GEORGINA; PALACIOS SARA
Revista:
Chemistry and Biodiversity
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2012
ISSN:
1612-1872
Resumen:
Previous studies determined that the ethanol extract from Flourensia oolepis S. F. Blake (Asteraceae) showed phytotoxic activity against Raphanus sativus seeds, at 10 mg/ml with a germination inhibition index (GI%) of 100%. In the present work, systematic bioassay-guided fractionation of the extract was performed to identify the phytotoxic compound. Two compounds, the flavanone pinocembrin and the sesquiterpene ilicol, were isolated as the active principle. Both compounds were active against the monocot seeds, P. miliaceum and Avena sativa, and the dicot seeds, R. sativus and Lactuca sativa. Pinocembrin was clearly the most active compound with an IC50 (germination) of 0.24, 3.40, 3.28 and 3.55 mM against P. miliaceum, A. sativa, L. sativa and R. sativus, respectively, while ilicol showed IC50 (germination) of 0.67, 2.73, 5.25 and 9.66 mM for the same species, respectively. Pinocembrin and ilicol also inhibited root growth, showing IC50 (root inhibition) of 0.199, 14.68, 8.05, 7.69 mM and 1.22, 2.90, 7.35, 8.07 mM, respectively, against P. miliaceum, A. sativa, L. sativa and R. sativus, respectively. Combination of both active compounds showed no potentiation effect against the four test seeds, indicating that there is no synergistic effect between them. Pinocembrin and ilicol reduced cell division of Allium cepa (onion) tips at 2 mM; this effect might explain the root-growth inhibitory properties observed for both compounds. However, no chromosome aberrations w