Mass spectrometry studies of Lycopodium alkaloid sauroine.

VALLEJO M; CIFUENTE D; CECATI F; ORTEGA M; CABRERA, JL.; MARTÍN V; TONN C; AGNESE AM; ARDANAZ C

RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM.

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2012 vol. 26 p. 1 - 6

0951-4198

Abstract:RATIONALE: Sauroine is a Lycopodium alkaloid. During the last years thesekinds of alkaloids has gained great interest for their complex moleculararchitectures as well as due to their acetylcholinesterase activity. Is knownthat drugs that inhibit acetylcholinesterase, can be used to treat earlystages of Alzheimer disease.METHODS: Saurione was isolated from aerial parts of Huperzia saururus(Lam.) Trevis. Electron ionization mass spectrometry (EI-MS) (LowResolution) and collision-induced dissociation tandem mass spectrometry intime (CID-MS/MS) fragmentation was conducted by an ion trap, GCQ Plusmass spectrometry with MS/MS. EI-high-resolution mass spectrometry(EI-HRMS) was performed in a magnetic sector mass spectrometerMicromass VG.RESULTS: Using GC/EI-CID-MS/MS we obtained the differentfragmentation routes that connect all the ionic populations. In addition, theuse of EI-HRMS allowed us to measure the exact masses of all thefragment ions, and, with all this information gathered, we tried to establisha fragmentation scheme concordant with the ascendant and descendantspecies.CONCLUSIONS: The mass spectrometry studies presented in this work canbe very useful for the structural determination of sauroine, when smallamounts of sample are available and to support structural determinationby NMR data.