Elimination of bacterial biofilms with an aerosol formulation of silver sulfadiazine

CARLOS RUBÉN MAS; ALEJANDRO MOYANO; ANDREA SMANIA; ANTONELLA COLQUE

Paraná

Congreso; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular, Paraná, Entre Ríos, Argentina.; 2018

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The healing processes of wounds in skin and soft tissues are permanently challenged by the risk of microbial infection, particularly in chronic wounds which are extremely susceptible of being colonized by gram-negative as well as gram-positive pathogens growing in a biofilm life-style. Dressings such as gauze, ointment or gels containing silver sulfadiazine have been developed to avoid infection and biofilm formation on wounds. In this study we test invitro an aerosol formulation of silver sulfadiazine (Platsul-AAEROSOL, Soubeiran Chobet SRL) for spray administration against biofilms of P. aeruginosa and against biofilms of two strains of S. aureus being resistant (MRSA) or sensible (MSSA) to methicillin. The spray allows the administration of the silver formulation without the direct contact with the wound, thereby avoiding possible cross-infection and trauma to the patient. Furthermore, the sealed container and the positive pressure by which the product is expelled avoids contamination ofthe product after reiterative usage. Thus, we evaluated this formulation on biofilms by carrying out biomass quantification by crystal violet staining assays, as well as viability measurements and live/dead confocal microscopy. This formulation displayed a strong antibiofilm effect, showing total bactericidal activity on biofilms of P. aeruginosa even after an 800-fold dilution of the product, and after a 100-fold and 10-fold dilution for MRSA andMSSA, respectively. Considering the benefits of a spray administration, our results support this kind of aerosol formulation as a potential improvement over conventional dressings of silver sulfadiazine.