Elimination of bacterial biofilms with an aerosol formulation of silver sulfadiazine

MOYANO, ALEJANDRO J.; SMANIA, ANDREA M.; MAS, CARLOS R.; COLQUE, CLAUDIA A.

Paraná

Congreso; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The healing processes of wounds in skin and soft tissues are permanently challenged by the risk of microbial infection, particularly in chronicwounds which are extremely susceptible of being colonized by gram-negative as well as gram-positive pathogens growing in a biofilm life-style.Dressings such as gauze, ointment or gels containing silver sulfadiazine have been developed to avoid infection and biofilm formation onwounds. In this study we test in vitro an aerosol formulation of silver sulfadiazine (Platsul-A AEROSOL, SoubeiranChobet SRL) for sprayadministration against biofilms of Pseudomonas aeruginosa and against biofilms of two strains of Staphylococcus aureus being resistant(MRSA) or sensible (MSSA) to methicillin. The spray allows the administration of the silver formulation without the direct contact with thewound, thereby avoiding possible cross-infection and trauma to the patient. Furthermore, the sealed container and the positive pressure by whichthe product is expelled avoids contamination of the product after reiterative usage. Thus, we evaluated this formulation on biofilms by carryingout biomass quantification by crystal violet staining assays, as well as viability measurements and live/dead confocal microscopy. Thisformulation displayed a strong antibiofilm effect, showing total bactericidal activity on biofilms of P. aeruginosa even after an 800-fold dilutionof the product, and after a 100-fold and 10-fold dilution for MRSA and MSSA, respectively. Considering the benefits of a spray administration,our results support this kind of aerosol formulation as a potential improvement over conventional dressings of silver sulfadiazine.