EFFECTS OF DIAZEPAM ON CD4 T CELLS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE)

FERNÁNDEZ HURST, NICOLÁS; ZANETTI SAMANTA R.; BOUZAT CECILIA B.; ROTH, GERMAN A.

Buenos Aires

Congreso; I Meeting LASID, FAIC and SAI; 2015

Sociedad Argentina de Inmunología (SAI)

CD4 T cells exert a critical role during the induction and development of EAE. Diazepam (Dz) is an allosteric agonist of the GABA-A receptor (GABA-AR) and also binds to the peripheral benzodiazepine receptor, TSPO. Previously, we demonstrated that Dz has a protective role during the development of EAE. In this study, we investigated how Dz mediates this action. The animals were sensitized with complete Freund?s adjuvant (CFA) alone as control group or with myelin to induce EAE, treated with various Dz concentrations, and sacrificed during the acute state of the disease. We evaluated the proliferation of isolated CD4 draining popliteal lymph node T cells in presence of Concanavalin A, Dz and Pk11195 (a specific antagonist of TPSO). Dz reduces the proliferation of CD4 T cells, Pk11195 partially blocks the effect of Dz only in CFA group (p