Evaluation of lactic acid bacteria as live vectors for oral DNA vaccines

RUSSI ROMINA; WAGNER PAULA; CAMUSSONE CECILIA; CALVINHO LUIS

Buenos Aires

Conferencia; IV LASID Meeting, LXIII Argentinean Immunology Society and II French-Argentinean Immunology Meeting; 2015

Novel strategies, such as development of oral/nasalvaccines administered by lactic acid bacteria can be thought as analternative for the induction of mucosal immunity. Nonpathogeniclactic acid bacteria (LAB) are an attractive alternativeas vehicles for delivery of DNA vaccines and other therapeuticmolecules into mucosa. The aim of this study was to assess twostrains of LAB as live vectors for oral DNA immunization againstβ-toxin from S. aureus and to characterize the humoral immuneresponse induced in mice. The coding sequence of β-toxin from S.aureus was inserted in an E. coli-LAB shuttle plasmid withkanamycin resistance (pLKV1-βtoxin). Lactobacillus paracaseiJP1 and Lactococcus lactis Mo12 were transformed with pLKV1-βtoxin by electroporation. 108 CFU of each recombinant strain ofLAB were orally administered to 5-6 weeks old, Balb/c femalemice every 24 hours for 3 consecutive days. The scheme wasrepeated 10 days after. Control animals received bacteriatransformed with plasmid without insert. Mice were sacrificed 10days after the last administration and sera and intestinal fluid (IF)were obtained. Anti-β-toxin antibodies were tested by ELISA inserum and in IF, using recombinant β-toxin. IgA in IF wassignificantly higher immunized compared to control L.lactis(p