MODIFICATION OF THE CELL CYCLE KINETICS IN C-FOS -/- NEURAL STEM/PROGENITOR CELLS

VELAZQUEZ FN; PRUCCA CG; DASTOLFO, DS; ETIENNE O; SILVESTRE, DO; BOUSSIN FD; CAPUTTO BL

Mar del Plata

Congreso; LI Reunion Anual del Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2015

SAIB

Neurons of the mammalian CNS are originated from progenitors dividing at the apical surface of theneuroepithelium. These cells show a high proliferation capacity and an adequate control of their growth is veryimportant. The protein c-Fos is known as an AP-1 transcription factor and as a protein that activate phospholipidsynthesis. c-fos -/- mice, although viable, die at approximately 7 months of age, are infertile and growth-retarded withrespect to their WT littermates. We know that the absence of c-fos in the developing cerebral cortex increases thenumber of apoptotic cells while reduced the number of differentiated cells. We determined the underlying bases ofthese differences. Neurospheres cultures show differences in the proliferation kinetics between c-fos -/- and c-fos +/+NSPCs. The distribution of these cells along the cell cycle indicated an increase in the S phase length and a reductionin the G1 phase. Dual injections of EdU and BrdU in c-fos-/- and c-fos+/+ E14.5 also showed an increased number ofcells at the S-phase in the embryonic cortical telencephalon of c-fos-/- mice, without changes in mitosis. These resultssugest that the absence of c-fos modified the length of the S and G1 phases of the cell-cycle of NSPCs, which couldbe the explanation for the differences in apoptosis and differentiation between c-fos +/+ and c-fos -/- mice.