Regulating the establishment of neuronal polarity by Growth factors

ALVARO NIETO GUIL; SANTIAGO QUIROGA

Huerta Grande, Córdoba

Congreso; XXVIII annual meeting of Chilean Society for Cell Biology; 2013

Sociedad Argentina de Investigación en Neurociencias.

Regulating the establishment of neuronal polarity by Growth factors   Álvaro Nieto, Santiago Quiroga     Universidad Nacional de Córdoba, Facultad de Ciencias Químicas Departamento de Química BiológicaCentro de Investigaciones en Química Biológica de Córdoba CONICET Av. Haya de la Torre s/n Ciudad Universitaria, Córdoba, Argentina     Neuronal polarity acquisition depends on axonal specification, whereby one of the undifferentiated neurites starts to grows rapidly and became an axon. The results obtained in our laboratory indicated that insulin-1 like growth factor (IGF-1) and its specific receptor would be essential for the establishment of neuronal polarity in cultured hippocampal cells (Sosa, Dupraz et al. 2006). Recently, however, a paper has been published in which signaling transforming growth factor beta 2 (TGF-β 2), specifies axons during brain development (Yi, Barnes et al. 2010). This has generated an interesting debate about the involvement of different growth factors in the regulation of axonal specification. The experiments designed in this project tend to re-examine the relationship of IGF-1 receptors and signaling pathways TGF-β2 in axon specification and establishment of polarity. After carrying out primary hippocampal culture and ?exo utero? or ?in utero? electroporation, the axonal development will be examined through profit or loss experiments receptor function of IGF-1 and TGF-β2. Therefore, new perspectives to this debate are expected to obtain.