New perspectives for the structural analysis of biomembrane models in South America

CAVALCANTI, LEIDE PASOS; RAFAEL GUSTAVO OLIVEIRA

San Javier, Tucumán

Congreso; XLI Reunión Anual de la Soc. Argentina de Biofísica; 2012

Soc. Argentina de Biofísica

Small angle x-ray scattering (SAXS) is a versatile technique applicable to the study of structural features of biomembrane vesicles and liposomes (as well as other non-lamellar phases) in isotropic suspension. More detailed information can be gained from anisotropic systems like Langmuir and Gibbs) monolayers at fluid/liquid interfaces [1-3], and planar multilayers on solid support [4]. This is because planar geometries allow for the splitting of the in-plane and out-of plane components of the scattering vector. The planar geometries also allow for further manipulations and simultaneous structural determinations under well defined thermodynamic conditions, like the isothermal lateral compression of monomolecular films in a Langmuir trough, as well as bulk compression (planar multilayers under osmotic stress). In this work, some examples of measurements with planar geometry are presented, and the possibility for their implementation at the LNLS is discussed. Referencias: 1. R. G. Oliveira, E. Schneck, B.E. Quinn, O.V. Konovalov, K. Brandenburg, U. Seydel, T. Gill, C.B. Hanna, D. A. Pink, M. Tanaka. C.R. Chim, 12 (2009) 209. 2. L. P. Cavalcanti, O. Konovalov, I. Torriani. Eur Biophys J 35 (2006) 431. 3. R. G. Oliveira, E. Schneck, B.E. Quinn, O.V. Konovalov, K. Brandenburg, T. Gutsmann, T. Gill, C.B. Hanna, D. A. Pink, M. Tanaka. Phys Rev E 81 (2010) 041901. 4. E. Schneck, R. G. Oliveira, F. Rehfeldt, B. Demé, K. Brandenburg, U. Seydel, M. Tanaka. Phys Rev E 80 041929. Acknowledgments: We thank Laboratorio Nacional de Luz Sıncrotron, Campinas, Brazil (beamline SAXS2), the Institute Laue Langevin and the European Synchrotron Radiation Facility (Grenoble) for experiment beamtimes. This work was supported by CONICET, Secretaría de Ciencia y Tecnología, University of Córdoba (SECyT-UNC). R.G.O is career investigator of CONICET (Argentina).