ARABIDOPSIS PROLINE DEHYDROGENASE PARTICIPATES IN THE ACTIVATION OF PATTERN TRIGGERED IMMUNITY

FABRO GEORGINA; ALVAREZ MARIA ELENA

Mendoza

Congreso; 48th Reunion Anual SAIB; 2012

Sociedad Argentina de Bioquimica y Biologia Molecular

Proline Dehydrogenase (ProDH) catalyzes the limiting step ofproline degradation in the mitochondria. In yeast and animal cells,ProDH hyperactivity contributes to imbalances in the production ofreactive oxygen species (ROS). In plants ProDH contributes toeffector triggered immunity (ETI) in response to pathogens. InArabidopsis ProDH is required for full development of theHypersensitive Response (HR), a localized cell-suicide reactionagainst pathogens that is signaled and stimulated by ROS.Arabidopsis genome encodes two ProDH genes: and .The aim of this work was to investigate if these genes are alsoinvolved in activation of defences triggered by recognition ofmicrobial patterns (Pattern Triggered Immunity, PTI). We usedbacterial flagellin (flg22) to evaluate PTI activation.We found thatflg22 induced an early and transient expression of but not. Single mutants for each gene showed reduced callosedeposition at the cell wall in response to the peptide. The growthcurves of a weakly virulent bacterial pathogen (DC3000 DeltaCEL) indicated that as well asmutants are hyper-susceptible to this strain, which is unable tosuppress PTI in wild type plants