The Immediate-Early oncoproteins Fra-1, c-Fos and c-Jun have distinguishable surface behavior and interactions with phospholipids

CECILIA GAGGIOTTI; DEL BOCA, MAXIMILIANO; CASTRO, GONZALO; CAPUTTO B.L,; BORIOLI, GRACIELA

BIOPOLYMERS

Wiley Interscience

Lugar: New Jersey; Año: 2009 vol. 91 p. 710 - 718

0006-3525

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> This work explores the surface properties of the transcription factor Fra-1 and compares them with those of two other Immediate Early proteins, c-Fos and c-Jun. We have gathered several experimental clues of the flexible nature of Fra-1, c-Fos and c-Jun that support sequence-based predictions of their intrinsical disorder. The values of surface parameters for Fra-1 are similar in general to those of c-Fos and c-Jun. However, we find differences in the interactions of the three proteins with phospholipids. The closely related Fra-1 and c-Fos share affinity for anionic lipids but the former has more affinity for a condensed phase and senses a change in DPPC phase, while the latter has more affinity for an expanded phase. c-Jun is not selective for phospholipid polar head group or charge.  Our view is that at least some Immediate Early transcription factors can interact with membrane phospholipids in a distinguishable manner, and this shall provide a basis for their potential capacity to regulate membrane-mediated cellular processes.