CIQUIBIC 05472
CENTRO DE INVESTIGACIONES EN QUIMICA BIOLOGICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Surface mixing of products and substrate of PLA in enzyme-free mixed monolayers reproduce enzyme-driven structural topography.
Autor/es:
DE TULLIO, L.; FANANI, M. L.; MAGGIO
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 1828 p. 2056 - 2063
ISSN:
0005-2736
Resumen:
It is well accepted that topographic changes in lipid monolayers hydrolized by lipolytic enzymes are a consequence of enzyme surface localization. Lateral packing deffects arrising from lipid phase coexistence are postulated places in which the enzyme is preferably localized. This work describes a method of mixing two lipid monolayers which simulate the manner that lipids are mixed when a lypolitic enzyme is hydrolizing the surface. In enzyme-free surface mixed film, a topographyc pattern similar to the generated actively by the enzyme was observed. The main conclusion from our experiments is that mixing-demixing properties of substrates and products of the enzymatic reaction determine topographyc evolution; enzyme surface localization is not the only factor.
