INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MILD STRESS INDUCED BY MATERNAL MANIPULATION DURING LATE GESTATION AND INFANTILE ETHANOL CONSUMPTION INDUCE CHANGES IN MU AND KAPPA OPIOID RECEPTORS, BUT NOT IN OPIOID LIGANDS PRECURSORS
Autor/es:
MACCHIONE, AF; ABATE P; GUTTLEIN L; MOLINA JC; MÉNDEZ UBACH M.
Lugar:
Puerto Varas
Reunión:
Congreso; VIII LASBRA INTERNATIONAL MEETING; 2017
Institución organizadora:
Latin American Society for Biomedical Research on Alcoholism (LASBRA)
Resumen:
Infant rats are vulnerable to ethanol?smotivational effects. Additionally, stressmediates sensitivity to ethanol?spostabsortive effects and consumption. Theimpact of mild stressors (i.e. maternalintubation during late gestation) remainsscarcely understood during early ontogeny.This work sought to characterize changes inopioid ligands precursors and receptors geneexpression in hypothalamus, after prenatalmanipulation and infantile ethanol intake.Dams received an intragastric administration(i.g) of ethanol (2g/kg), water or wereundisturbed, during gestational days 17-20.At postnatal days (PDs) 14-15, pups wereevaluated in terms of 0% or 5% ethanolconsumption. A third untreated group wasadded. After intake test (PD15), mRNA levels of opioid ligands precursors and receptorswere measured, by real time PCR. The mRNAexpression of ligand precursors did notappear to be affected by either, prenatalmanipulation or postnatal intake of the drug.However, the mRNA expression of mu andkappa receptors seems to be sensible toprenatal mild stress and ethanol intake.Infantile ethanol intake seemed to upregulatethe expression of mu and kappareceptors in naïve pups. Nevertheless, ethanolconsumption (PDs14-15) resulted in a downregulationof opioid receptors when pupswere exposed to mild prenatal stress. Ourresults suggest that the induction of a mildstress during late gestation is enough tomake relatively permanent changes in muand kappa expression on hypothalamus,when pups have the opportunity to intake thedrug. This work was supported by grants fromANPCyT (PICT 2011-0130) CONICET andSECyT-UNC.