INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Pseudomonas aeruginosa CDF transporters CzcD and YiiP are involved in Zn2+ efflux, outer membrane permeability and antibiotic resistance.
Autor/es:
ELSO-BERBERIÁN G; SALUSSO A; RAIMUNDA D
Lugar:
Rosario
Reunión:
Congreso; XXIII CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA - XIV CONGRESO ARGENTINO DE MICROBIOLOGIA; 2016
Resumen:
Transporters of the cation diffusion facilitator family form dimers that export transition metals from the cytosol. The opportunistic pathogen Pseudomonas aeruginosa presents three homologous CDF genes, czcD, cotP and yiiP. CotP appears to have a role in Co2+ homeostasis as the deletion mutant strain ΔcotP is more sensitive and accumulate this ion. In inhibition halo experiments, ΔczcD and ΔyiiP strains showed low Zn2+sensitivity. However, in iron‐rich media and in the presence of Zn2+ the strains secreted higher levels of the iron chelator pyoverdine. The nonpyoverdineproducer double mutants, ΔpvdA‐ΔczcD and ΔpvdA‐ΔyiiP, displayed increased Zn2+ sensitivity compared to the single mutant ΔpvdA. Most importantly, the strains ΔczcD and ΔyiiP showed a 4‐ to 8‐fold increased sensitivity to imipenem and ciprofloxacin and a 2‐fold increased sensitivity to chloramphenicol and gentamycin. These observations correlated with increased sensitivity to EDTA and overexpression of OprN and OprD porins in ΔczcD and ΔyiiP. We hypothesize that CzcD and YiiP function as heterodimer exporting Zn2+ to the periplasm and that their function is required for outer membrane stability and therefore antibiotic tolerance while CotP is a Co2+ efflux transporter.