INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differential prolonged effects of chronic variable stress in adolescence and adulthood
Autor/es:
SCARPONI, A; COTELLA, E. M.; PAGLINI, MG; HERMAN, JP
Lugar:
Newport
Reunión:
Workshop; Neurobiology of Stress Workshop; 2016
Resumen:
Adolescent animals are known to be highly vulnerable to the effects of adverse environment. It is known that adolescent rat males hyper-respond to chronic variable stress (CVS). Considering that chronic stress can elicit long-term effects, we hypothesized that adolescent chronic stress causes life-long impairment in the behavioral and physiological adaptations to stressful experiences, and are more pronounced that the long term effects of chronic stress during adulthood. Materials and methods. Wistar rats PND40 (Adol) and PND60 (Adult) were subjected to CVS for 2 weeks, with stressors presented randomly twice daily and twice a week overnight stressors consisted of: 1 h cold room (4 °C), 30 min hypoxia (oxygen 8% ,nitrogen 92%) 1 h shaker stress (100 rpm), 2h individual housing in metabolic cages, 5 min new environment exposure, 0.15M LiCl i.p. injection overnight social isolation (one rat/cage), overnight social crowding (six rats/cage). Controls underwent only normal animal handling. 5 weeks after CVS, animals were evaluated using the elevated plus maze (EPM) and the forced swim test. The HPA (spell out) axis response after 30 min restraint was also assessed. Results: Compared to controls, rats that were subjected to CVS during adolescence showed less total arm activity when subjected to the EPM test in adulthood (p