Maternal exposition to NaCl solution modifies fluid balance restoration in the offspring

MACCHIONE AF; VIVAS L

Mendoza

Congreso; XXVI Reunión Científica Anual Sociedad de Biología de Cuyo.; 2008

It has been reported that pregnancy history affects sodium homeostasis in adult offspring and might have consequences, like taste-driven alterations in NaCl intake. The experimental paradigm of partial aortic ligation (PAL) alters pregnancy environment through the endogenous activation of renin-angiotensin-aldosterone system. We studied sodium and water intake and brain pattern of Fos immunoreactivity (Fos-ir) in  adult offspring from rats that had been treated with 3 different experimental conditions: I) PAL: Ligation/0.45M NaCl/water access, II) SHAM: Sham ligation/0.45M NaCl/water access, III)CONTROL: Sham ligation/water access only. The offspring were sodium depleted by a combined treatment of sc furosemide (20mg/kg) and sodium-free diet,  which induces hypovolemic thirst. The control group consumed larger amounts of water than PAL and SHAM groups. Furthermore, PAL offspring consumed smaller amounts of 0.45M NaCl than Sham or Control groups (MANCOVA, p < 0.05). Results also indicated a substantially lower activation  within the supraoptic nucleus in PAL group vs the other groups, as shown by Fos-ir. In conclusion, our data demonstrate a long-term effect of maternal NaCl exposure, altering mechanisms that regulate fluid balance in the offspring. Supported by ANPCyT and CONICET.