INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Agonist mobility in supported bilayers affects Fas mediated death response
Autor/es:
SANCHEZ MARIA FLORENCIA; LEVI VALERIA; THOMAS WEIDEMANN; DOLORES C. CARRER
Lugar:
Foz de Iguazú
Reunión:
Congreso; 23rd Congress of the International Union for Biochemistry and Molecular Biology (IUBMB) and 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq).; 2015
Institución organizadora:
Int. Union Biochem. Molec. Biol. y Brazilian Society for Biochemistry and Molecular Biology
Resumen:
Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. SinceFas-mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer.