• Protein C4 is a putative small heat shock protein in the intestinal parasite Giardia lamblia

NORES, MJ; GOTTIG, N; PRUCCA, C; CAVALLÍN, L; QUIROGA, R; CARRANZA, P; SOLARI, A; LUJÁN, HD

Mar del Plata, Argentina.

Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2007

G. lamblia is a medical important protozoan with a low position in the eukaryotic lineage. trophozoites are transmited through infected hosts by differentiation into cyst forms. By screening a WB 1267 cDNA expression library, we cloned the previously isolated gene Glorf-C4, considered specific of this organism. It encodes a 22 kDa protein that assembles in 60-66 kDa and high molecular mass complexes during the whole life cycle. 66 kDa complex formation is favored under some stress conditions. C4 localizes in cytosol of trophozoites and cysts and forms large spherical aggregates when it is overexpressed, without affecting viability and encystation. No homologous proteins nor conserved domains or features arise from sequence analysis of the predicted protein. Nevertheless, C4 presents local tertiary structure similarity with the a–crystallin domain of small heat shock proteins Hsp16.9B from and Hsp16.5 from the hyperthermophilic archaea . Here, we discuss C4 putative role as a small heat shock protein in biology.