Activacion y muerte neuronal en la AMEX en el modelo de epilepsia con Acido Kainico

PERENO, G; BALASZCZUK, V; BELTRAMINO, C

Buenos Aires

Congreso; IX Congreso Argentino de Neuropsiquiatría y 5to Congreso Latinoamericano de Neuropsiquiatria y 10a Jornadas de Enf. de Alzheimer y Trastornos Cognitivos; 2007

Asoc. Argentina de Neuropsiquiatria.

The Medial Extended Amygdala (MExA) integrates an anatomic continuum between the Medial Nucleus and the Bed Núcleos of  the Stria Terminalis, modulating the pheromonal perception, and emotional and social behaviors. Objective: to study the response of MExA to Kainic Acid, drug used as animal model of Temporal Lobe Epilepsy (TLE), and its neuronal activation by Fos and Fos B,  and the probable occurrence of neuronal death. Methods: twelve adult Wistar male rats were used. Six animals received a single IP injection of KA (8 mg/kg), and six (controls) were injected  with saline. After 2-72 hours of survival, the animals were fixed with paraformaldehide in buffer. The brains were sectioned serially and stained for fos and fos B in parallel series. Other series were stained to reveal neuronal death with the Amino-Cupro-Argentic technique.  Nuclei (fos, fosB) and neurons (Ag) were counted by a Scion Program (NIH). Data were analysed using  ANOVA followed by the Fisher post hoc Test. Results: numerous nuclei reactive to fos and fos  B  were found in MExA after KA injection, being it´s expression different between them and from control animals.  P< 0,05.  Fos decrease and fosB increase from 2 to 72 hours of experimental period.  Neuronal death paralleled the fos B reaction. Conclusions: we temonstrate that Kainic Acid activates early genes fos and fosB in neurons of the MExA  indicating the vulnerability of this structure to TLE.