INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Adaptor Protein 2: an essential player in Giardia lysosomal protein trafficking.
Autor/es:
RIVERO MR; VRANYCH CV; ROPOLO AS; TOUZ MC
Lugar:
Mar del Plata. Buenos Aires.
Reunión:
Congreso; 43rd Annual Meeting. Argentine Society for Biochemistry and Molecular Biology Research.; 2007
Institución organizadora:
Argentine Society for Biochemistry and Molecular Biology Research.
Resumen:
In mammalian cells, the role of clathrin and adaptor protein complexes (APs) in endosomal and lysosomal protein delivery is well known. Unlike these cells, only AP1 and AP2 are present in the Giardia genome. We recently found that gAP1 participates in lysosomal protein trafficking from a sorting organelle to the lysosomal-like peripheral vacuoles (PVs) in Giardia, but the function of AP2 had not been elucidated. Based on what was described for more evolved cells, we hypothesize that gAP2 is the adaptor protein involved in vesicular trafficking from the plasma membrane to the PVs. By using a specific antibody against one of the subunits of gAP2 (ì2), we showed that it localizes in the PVs as well as close to the plasma membrane. To analyze the interaction of gAP2 with associated proteins the YTH and CoIPP assays were performed. Production of ì2 ds RNA for protein knock-down was used to study the role of gAP2 in receptor-dependent and independent endocytic mechanisms. By using different approaches we were able to observe that gAP2 is critical in trophozoite growth and differentiation and that depletion of ì2 affects receptor-mediated endocytosis. The complete characterization of gAP2 role would contribute to increase our knowledge about this extremely simplified protein trafficking system present in this primitive human parasite.Giardia genome. We recently found that gAP1 participates in lysosomal protein trafficking from a sorting organelle to the lysosomal-like peripheral vacuoles (PVs) in Giardia, but the function of AP2 had not been elucidated. Based on what was described for more evolved cells, we hypothesize that gAP2 is the adaptor protein involved in vesicular trafficking from the plasma membrane to the PVs. By using a specific antibody against one of the subunits of gAP2 (ì2), we showed that it localizes in the PVs as well as close to the plasma membrane. To analyze the interaction of gAP2 with associated proteins the YTH and CoIPP assays were performed. Production of ì2 ds RNA for protein knock-down was used to study the role of gAP2 in receptor-dependent and independent endocytic mechanisms. By using different approaches we were able to observe that gAP2 is critical in trophozoite growth and differentiation and that depletion of ì2 affects receptor-mediated endocytosis. The complete characterization of gAP2 role would contribute to increase our knowledge about this extremely simplified protein trafficking system present in this primitive human parasite.Giardia, but the function of AP2 had not been elucidated. Based on what was described for more evolved cells, we hypothesize that gAP2 is the adaptor protein involved in vesicular trafficking from the plasma membrane to the PVs. By using a specific antibody against one of the subunits of gAP2 (ì2), we showed that it localizes in the PVs as well as close to the plasma membrane. To analyze the interaction of gAP2 with associated proteins the YTH and CoIPP assays were performed. Production of ì2 ds RNA for protein knock-down was used to study the role of gAP2 in receptor-dependent and independent endocytic mechanisms. By using different approaches we were able to observe that gAP2 is critical in trophozoite growth and differentiation and that depletion of ì2 affects receptor-mediated endocytosis. The complete characterization of gAP2 role would contribute to increase our knowledge about this extremely simplified protein trafficking system present in this primitive human parasite.ì2 ds RNA for protein knock-down was used to study the role of gAP2 in receptor-dependent and independent endocytic mechanisms. By using different approaches we were able to observe that gAP2 is critical in trophozoite growth and differentiation and that depletion of ì2 affects receptor-mediated endocytosis. The complete characterization of gAP2 role would contribute to increase our knowledge about this extremely simplified protein trafficking system present in this primitive human parasite.