EFFECTS OF PRENATAL ETHANOL EXPOSURE ON HIDROELECTROLYTE HOMEOSTASIS

A. GODINO; VIVAS L.; MOLINA JC

Congreso; SSIB 2014, the 22nd Annual Meeting of the Society for the Study of Ingestive Behavior; 2014

Abstract# 127 The aim of the present work was to analyze the effect of prenatal exposure to moderate doses of ethanol (Pre-EtOH) during the gestational days (GD) 17 to 20 on offspring (postnatal day 28-33) hydroelectrolyte balance. We analyzed basal brain neural activity and basal/induced sodium appetite and renal response stimulated by sodium depletion (SD). Wistar pregnant dams received one daily intragastric administration of 0 or 2 g/kg/ml. SD was induced by Furosemide and low sodium diet treatment (FURO +LSD). Another group was submitted to immunohistochemical detection of Fra like (Fra-LI-ir) protein and oxytocin (OT) and/or vasopressin (AVP). Pre-EtOH group reduced water intake (1.4±0.3 vs 2.5±0.3; n=16) but did not affect induced sodium intake. Basal Na and K concentration of 24 h urine were reduced in Pre-EtOH pups (Na: 125.03 ±7.06 n=10 vs 101.2 ± 5.97 n=14; K: 98.6±6.32 vs 76.98 ± 5.39); besides the renal response after SD did not change. However, the correlation between urinary volume and water intake induced by FURO was significantly different (Pre-EtOH vs control). At brain level the number of Fra-LI-ir was significantly increased in AVP magnocellular neurons of Paraventricular nucleus (PVN) (PaLM: 19.4 ±5.3 vs 39.2 ±7.3 n=5 and PaMM: 9,6 ±2.3 vs 19 ±2.4 n=3) in Pre-EtOH pups. We did not observe any difference in Fra-LI positive cells along OT neurons. In summary, Pre-EtOH exposure alters hydroelectrolyte balance regulation, since modulated induced water intake and renal responses, suggesting a reduced capacity to retain body water, possibly mediated by the chronically increased activity of AVP PVN neurons.