INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression of key steroidogenic enzymes in developing brain: hormonal compensation of sex chromosomes-induced sex differences
Autor/es:
CISTERNAS, C.D.; TOMÉ, K; CAMBIASSO, M.J.
Lugar:
Huerta Grande Cordoba
Reunión:
Congreso; XXIV Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2014
Institución organizadora:
SAN
Resumen:
EXPRESSION OF KEY STEROIDOGENIC ENZYMES IN DEVELOPING BRAIN: HORMONAL COMPENSATION OF SEX CHROMOSOMES-INDUCED SEX DIFFERENCES  Cisternas CD, Tome K, Cambiasso MJ Instituto Ferreyra, INIMEC-CONICET-Universidad de Córdoba. Córdoba-Argentina   The aim of this study was to investigate the brain expression of key steroidogenic enzymes and the mitochondrial cholesterol transporter Star in transgenic mice in which the effect of gonadal sex (testes vs. ovaries) and sex chromosome complement (XX vs. XY) are dissociated. We have evaluated by qPCR the expression of star (steroidogenic acute regulatory protein), cyp11a1 (cytochrome P450-side chain cleavage), cyp19a1 (cytochrome P450-aromatase), srd5a1 and srd5a2 (5 alpha reductase I and II) in the stria terminalis (ST), anterior amygdaloid area (AAA) and amygdala of E16 mouse brain. ANOVA indicated that cyp11a1, star, srd5a1 and srd5a2 did not differ between genotypes in these brain areas. However, XY mice showed higher expression levels of cyp19a1 than XX (P<0.05) independently of gonadal sex in ST and AAA. Next, we have evaluated the effect of E2 (10-10M) and dehydrotestosterone (DHT, 10-10M) on cyp19a1 expression in neuronal amygdala cultures. ANOVA revealed a significant interaction between genotype and treatment (P<0.05). E2 and DHT increased aromatase levels in XX cultures (males and females) to similar levels of XY. These results suggest that gonadal steroids could compensate differences induced by sex chromosomes in aromatase expression early in mouse brain development.