INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Poster: Characterization of membrane lipids and protein lateral diffusion in patterned surfaces
Autor/es:
SANCHEZ MARIA FLORENCIA; KUNIK, D.; LEVI VALERIA; CARRER, D. C.
Lugar:
San Diego
Reunión:
Congreso; 56th Annual Meeting Biophysical Society; 2012
Institución organizadora:
Biophysical Society (EEUU)
Resumen:
The construction of functionalized surfaces for uses in nanotechnology and biotechnology has opened a new area in materials science. Advances in microfabrication and nanofabrication processes are also opening new opportunities to investigate complex questions in Cell Biology in a way not previously possible. In particular, supported lipid bilayers (SLBs) are popular models of membranes with potential bio-technological applications. Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure. Micro Contact Printing is a well-known and useful way to pattern a wide range of molecules into micro-scale, for example patches of lipidic membranes. Fluorescent dyes added to UV-cure resins allow the rapid fabrication of fluorescent micropatterns on standard glass coverslips by two-photon optical lithography. Furthermore, the high-relief topology of the lithographed substrates is utilized as a mold for poly (dimethylsiloxane) stamps to create patterns by micro contact printing, representing an alternative to the current etching techniques. Fluorescence correlation spectroscopy (FCS) is a powerful technique to study the lateral organization of membranes. It measures fluorescence intensity fluctuations in the single molecule regime and allows the determination of diffusion coefficients. In this work we present our progress in the preparation and characterization of supported membranes with controlled topologies and ability to bind ligands of interest, in particular, streptavidin and a biotinylated antibody. The lateral diffusion coefficients of the different components in each patterned surface were determined by FCS.