Conferencia por invitación: Nanoparticles in dermal drug delivery: effect of skin architecture

CARRER, D. C.; FREITES, L.

Potrero de los Funes

Simposio; 2do Simposio Latinoamericano de Nanomedicinas; 2012

Asociacion Argentina de Nanomedicina

Introduction: The knowledge of skin architecture and its impact on nanoparticles penetration is of great importance for the rational design of nanoparticles in the dermal/transdermal drug delivery field. We have shown before (Carrer et al. 2008) in mammal skin (pig) that two photon microscopy reveals the skin as organized into columns or groups of cells, separated by structures that we called canyons. The canyons start in the surface and reach the Stratum Basale, very near the epidermis/dermis plane. According to our observations, these structures appear to be an invagination or an extension of the Stratum Corneum. The canyons are also the preferred way for ultraflexible liposomes/liposomal-incorporated material to penetrate. In this work we have studied by confocal microscopy the penetration of ultraflexible liposomes, archeosomes and dendrimers into human skin. Results: We confirmed the presence of a skin organization in human skin similar to what we had observed in pig. We measured by confocal microscopy the penetration of a fluorescent protein included in the aqueous compartment of liposomes and archeosomes and compared it to the penetration of two different fluorescently labeled dendrimers. Conclusions: Human skin architecture appears to be similar to that observed in pig, with cell clusters separated by canyons. The ultraflexible liposomes penetrate deeper than the other formulations. All of the formulations penetrate up to higher depths through the canyons as compared to the cell clusters. Quantitative asessments of penetration must take into account the kind of skin structure in which the particles prefer to partition and penetrate References: 1-     Carrer D.C., Vermehren C., Bagatolli L. A. (2008) Pig skin structure and transdermal delivery of liposomes: a two photon microscopy study. J. Controlled Release 132: 12-20.