INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Conferencia por invitación: Nanoparticles in dermal drug delivery: effect of skin architecture
Autor/es:
CARRER, D. C.; FREITES, L.
Lugar:
Potrero de los Funes
Reunión:
Simposio; 2do Simposio Latinoamericano de Nanomedicinas; 2012
Institución organizadora:
Asociacion Argentina de Nanomedicina
Resumen:
Introduction: The knowledge of skin architecture and its impact on
nanoparticles penetration is of great importance for the rational design of
nanoparticles in the dermal/transdermal drug delivery field. We have shown
before (Carrer et al. 2008) in mammal skin (pig) that two photon microscopy
reveals the skin as organized into columns or groups of cells, separated by
structures that we called canyons. The canyons start in the surface and reach
the Stratum Basale, very near the epidermis/dermis plane. According to our
observations, these structures appear to be an invagination or an extension of
the Stratum Corneum. The canyons are also the preferred way for ultraflexible
liposomes/liposomal-incorporated material to penetrate. In this work we have
studied by confocal microscopy the penetration of ultraflexible liposomes,
archeosomes and dendrimers into human skin.
Results: We confirmed the presence of a skin organization in
human skin similar to what we had observed in pig. We measured by confocal
microscopy the penetration of a fluorescent protein included in the aqueous
compartment of liposomes and archeosomes and compared it to the penetration of
two different fluorescently labeled dendrimers.
Conclusions: Human skin architecture appears to be similar to
that observed in pig, with cell clusters separated by canyons. The
ultraflexible liposomes penetrate deeper than the other formulations. All of
the formulations penetrate up to higher depths through the canyons as compared
to the cell clusters. Quantitative asessments of penetration must take into
account the kind of skin structure in which the particles prefer to partition
and penetrate
References:
1- Carrer D.C., Vermehren C., Bagatolli L. A. (2008) Pig
skin structure and transdermal delivery of liposomes: a two photon microscopy
study. J. Controlled Release 132: 12-20.