INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Rol of Protein Kinase D1 in Group I glutamate metabotropic receptors trafficking.
Autor/es:
MASSERONI L.; GASTALDI, L; JAUSORO, I; QUASSOLLO, G; CORIA S.; DIAZ ANEL A.
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2012
Institución organizadora:
SAIB
Resumen:
Most of neuronal dendritic and axonal membrane protein sorting, a key
process for establishment and maintenance of neuron polarity, occurs at
the Trans-Golgi Network (TGN).
Metabotropic glutamate receptors belong to G protein-coupled receptor
(GPCR) family of seven-transmembrane domain; they contribute to
synaptic plasticity, hence in learning and memory processes. Their
transport towards their final localization, dendrites or axons, is still a
complex and not well-characterized process.
Protein Kinase D1 (PKD1) is a major component in membrane
trafficking events; in neurons, PKD1 participates in dendritic membrane
proteins sorting, such as transferrin receptor (TfR).
These previous observations lead us to hypothesize that PKD1
regulates Group I metabotropic glutamate receptors (mGluR) sorting,
since they are dendritic membrane proteins. Using 14 DIV hippocampal
neurons, we evaluate the effect in the mGluR1 and mGluR5 intracellular
trafficking from the TGN to proper dendritic domain by using: i)
depletion of the PKD1 by short harpin RNA, ii) a dominant negative
mutant (PKD1 kinase dead), and iii) chemical inhibitors.
Taking together, the results showed that the two receptors are
missorted and inserted in both dendritic and axonal membranes.
Moreover, we proved that the signaling pathway whereby PKD1 is
activated in neurons was through Phospholipase C (PLC) and Protein
kinase C (PKC)