Poster: Characterization of membrane lipids and protein lateral diffusion in patterned surfaces

SANCHEZ MARIA FLORENCIA; DODES TRAIAN M.; LEVI VALERIA; CARRER, D. C.

Buenos Aires

Workshop; 3rd South American Workshop on new trends in advanced fluorescence microscopy techniques; 2011

Univ. de Buenos Aires

The construction of functionalized surfaces for uses in nanotechnology and biotechnology has opened a new area in materials science. Advances in microfabrication and nanofabrication processes are also opening new opportunities to investigate complex questions in Cell Biology in a way not previously possible. In particular, supported lipid bilayers (SLBs) are popular models of membranes with potential bio-technological applications. Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure. Fluorescence correlation spectroscopy (FCS) is a powerful technique to study the lateral organization of membranes. It measures fluorescence intensity fluctuations in the single molecule regime and allows the determination of diffusion coefficients. In this work we present our progress in the use of the technique of Micro Contact Printing and results obtained on the modification of the topology of supported membranes and the ability of the membrane to bind ligands of interest, in particular, streptavidin and a biotinilated Antibody. Finally, the lateral diffusion coefficients of the different components in each patterned surface were determined by z-scan FCS method.